Laboratory Biosafety Guidelines

3rd Edition 2004

Laboratory Biosafety Guidelines (PDF Document 563 KB - 125 pages)

Table of Contents

• Editorial Committee and Acknowledgements
• Abbreviations
• Chapter 1
  Introduction
• Chapter 2
  Biological Safety
  • 2.1 Risk Groups
  • 2.2 Containment Levels
  • 2.3 Risk Assessment
  • 2.4 Health and Medical Surveillance
  • 2.5 Management of Biological Safety
  • 2.6 Biosecurity
• Chapter 3
  Handling Infectious Substances
  • 3.1 Operational Practices for Laboratories
    • 3.1.1 General Practices
    • 3.1.2 Containment Level 2
    • 3.1.3 Containment Level 3
    • 3.1.4 Containment Level 4
• Chapter 4
  Laboratory Design and Physical Requirements
  • 4.1 Matrix 1: Laboratory Location and Access
  • 4.2 Matrix 2: Surface Finishes and Casework
  • 4.3 Matrix 3: Heating, Ventilation and Air Conditioning
  • 4.4 Matrix 4: Containment Perimeter
  • 4.5 Matrix 5: Laboratory Services
• Chapter 5
  Commissioning, Certification and Recertification for CL3 and CL4 Laboratories
  • 5.1 Introduction
    • 5.1.1 Commissioning
    • 5.1.2 Certification
    • 5.1.3 Recertification
  • 5.2 Room Integrity
    • 5.2.1 Matrix 6: Room Integrity
    • 5.2.2 Room Pressure Decay Testing
  • 5.3 Air Handling Systems
    • 5.3.1 Matrix 7: Air Handling Systems
  • 5.4 Matrix 8: Laboratory Equipment and Services
• Chapter 6
  Large Scale Production of Microorganisms
  • 6.1 Introduction
  • 6.2 Scope
  • 6.3 Operational Practices and Physical Requirements
    • 6.3.1 Containment Level 1 Large Scale
    • 6.3.2 Containment Level 2 Large Scale
    • 6.3.3 Containment Level 3 Large Scale
• Chapter 7
  Program Specific Guidelines
  • 7.1 Laboratory animals
    • 7.1.1 General Requirements
    • 7.1.2 Non-human Primates
  • 7.2 Recombinant DNA and Genetic Manipulation 
  • 7.3 Cell Lines
    • 7.3.1 Risk Assessment
    • 7.3.2 Contamination with Infectious Agents
    • 7.3.3 Self-Self Experiments
• Chapter 8
  Decontamination
  • 8.1 Introduction
  • 8.2 Autoclaves
  • 8.3 Chemical Disinfection
  • 8.4 Gaseous Decontamination of Rooms
  • 8.5 Liquid Effluent Treatment Systems
  • 8.6 Irradiation
  • 8.7 Incineration
  • 8.8 New Technologies
• Chapter 9
  Biological Safety Cabinets
  • 9.1 Introduction
  • 9.2 Classes and Characteristics of Biological Safety Cabinets
  • 9.3 Installation and Certification
  • 9.4 Use of the Cabinet
• Chapter 10
  Regulatory Aspects for Handling Infectious Substances
  • 10.1 Importation and Transfer of Human Pathogens
  • 10.2 Export of Pathogens
  • 10.3 Transportation
  • 10.4 Importation, Transfer, and Containment of Animal Pathogens