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Laboratoire national d'immunologie du VIH (LNIV)

Le Laboratoire national, immunologie du VIH, diffuse de l'information par la publication d'articles dans des journaux approuvés par les pairs et la présentation de conférences et d'affiches lors de rencontres nationales et internationales.

Articles revus par un comité de lecture

  1. Bergeron M, Faucher S, Minkus T, Lacroix F, Ding T, Phaneuf S, Somorjai R, Summers R, Mandy F. Impact of unified procedures as implemented in the Canadian Quality Assurance Program for T lymphocyte subset enumeration. Participating Flow Cytometry Laboratories of the Canadian Clinical Trials Network for HIV/AIDS Therapies. Cytometry 1998;33(2):146-55.

  2. Brando B, Barnett D, Janossy G, Mandy F, Autran B, Rothe G, Scarpati B, D'Avanzo G, D'hautcourt JL, Lenkei R, Schmitz G, Kunkl A, Chianese R, Papa S, Gratama JW. Cytofluorometric methods for assessing absolute numbers of cell subsets in blood. Cytometry 2000;42(6):327-46.

  3. Gratama JW, D'hautcourt JL, Mandy F, Rothe G, Barnett D, Janossy G, Papa S, Schmitz G, Lenkei R. Flow cytometric quantitation of immunofluorescence intensity: problems and perspectives. European Working Group on Clinical Cell Analysis. Cytometry 1998;33(2):166-78.

  4. Lenkei R, Gratama JW, Rothe G, Schmitz G, D'hautcourt JL, Arekrans A, Mandy F, Marti G. Performance of calibration standards for antigen quantitation with flow cytometry. Cytometry 1998;33(2):188-96.

  5. Mandy F, Bergeron M, Izaguirre C. Application tools for clinical flow cytometry: Gating parameters for immunophenotyping. Clin Immunol Newsletter 1992;12(2):25-32.

  6. Mandy F, Brando B. Enumeration of absolute cell counts using immunophenotypic techniques. In: Robinson JP, Darzynkiewicz Z, Dean PN, Dressler LG, Rabinovitch PS, Stewart CC, Tanke HJ, Wheeless LL, editors. Current protocols in cytometry, Supplement. 13 ed. New York: John Wiley & Sons, 2000:6.8.1-6.8.26.

  7. Mandy FF, Bergeron M, Recktenwald D, Izaguirre CA. A simultaneous three-color T cell subsets analysis with single laser flow cytometers using T cell gating protocol. Comparison with conventional two-color immunophenotyping method. J Immunol Methods 1992;156(2):151-62.

  8. Mandy FF, Bergeron M, Minkus T. Principles of flow cytometry. Transfus Sci 1995;16(4):303-14.

  9. Mandy FF, Bergeron M, Minkus T. Evolution of leukocyte immunophenotyping as influenced by the HIV/AIDS pandemic: a short history of the development of gating strategies for CD4+ T-cell enumeration. Cytometry 1997;30(4):157-65.

  10. Nicholson J, Kidd P, Mandy F, Livnat D, Kagan J. Three-color supplement to the NIAID DAIDS guideline for flow cytometric immunophenotyping. Cytometry 1996;26(3):227-30.

  11. Nicholson JKA, Mandy FF. Immunophenotyping in HIV infection. In: Stewart C, Nicholson JKA, editors. Immunophenotyping. 1st ed. New York: Wiley-Liss, 2000:261-89.

  12. O'Gorman MR, Gelman R. Inter- and intrainstitutional evaluation of automated volumetric capillary cytometry for the quantitation of CD4- and CD8-positive T lymphocytes in the peripheral blood of persons infected with human immunodeficiency virus. Site Investigators and the NIAID New CD4 Technologies Focus Group. Clin Diagn Lab Immunol 1997;4(2):173-79.

  13. Prud'homme IT, Kim JE, Pilon RG, Minkus T, Hawley-Foss N, Cameron W, Rud EW. Amplicor HIV monitor, NASBA HIV-1 RNA QT and quantiplex HIV RNA version 2.0 viral load assays: a Canadian evaluation. J Clin Virol 1998;11(3):189-202.

  14. Schnizlein-Bick CT, Spritzler J, Wilkening CL, Nicholson JK, O'Gorman MR. Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults. Site Investigators and The NIAID DAIDS New Technologies Evaluation Group. Clin Diagn Lab Immunol 2000;7(3):336-43.

  15. Zenger VE, Vogt R, Mandy F, Schwartz A, Marti GE. Quantitative flow cytometry: inter-laboratory variation. Cytometry 1998;33(2):138-45.

Articles publiés dans des journaux internationaux sans comité de lecture

  1. Lenkei R, Marti G, Vogt R, Nicholson J, Mandy F. Meeting Summary: EC, FDA, and Health Canada. Cytometry (Communications in Clinical Cytometry) 1998;33(2):94.

  2. Lenkei R, Mandy F, Marti G, Vogt R. Preface. Cytometry (Communications in Clinical Cytometry) 1998;33(2):93.

  3. Mandy F. Abstracts from the 8th Canadian Flow Cytometry Consensus Meeting, November 16-18, 1997, Aylmer, Quebec, Canada. Cytometry (Communications in Clinical Flow Cytometry) 1998;34(1):50.

  4. Mandy F. Abstracts from the 9th Canadian Flow Cytometry Consensus Meeting, October 25-27,1998, Mont Ste.Anne,Quebec, Canada. Cytometry (Communications in Clinical Cytometry) 1999;38(2):81-82.

  5. Mandy F. An introduction to a unique review article. Cytometry (Communications in Clinical Cytometry) 1999;38(1):1.

  6. Mandy F, Bergeron M, Minkus T. Current activities and directions on the use of flow cytometry in HIV disease. Canadian Hematology Society Newletter, April, 1998.

  7. Montpetit M, Gleeson T, Mandy F. Flow molecular cytobiology: The next generation? Clin Immunol Newsletter 1998;18(11/12).

Résumés publiés

  1. Bagwell CB, Stelzer GT, Purvis N, Schwartz A, Stewart C, Mandy F. Multiple intensity method for compensating listmode data. Cytometry 1996;8(Suppl):119.

  2. Bergeron M, Lacroix F, Minkus T, Chabot C, Phaneuf S, Somorjai R, Mandy F. Comparison of single versus double platform absolute count determination of T-cell subsets by flow cytometry. XI International Conference on AIDS, Vancouver. 1996:255. Abstract 4008).

  3. Bergeron M, Lacroix F, Minkus T, Chabot C, Phaneuf S, Somorjai R, Mandy F. Evaluation of absolute count determinations of CD4 T-cells in HIV infection utilizing various single and double platform based cytometric methods. Cytometry 1996;8(Suppl):77.

  4. Bergeron M, Phaneuf S, Ding T, Minkus T, Mandy F. Single PLatform Technology: To evaluate inter-laboratory variation. Can J Infect Dis 1997;8(Suppl A):74A.

  5. Bergeron M, Mandy F, Ding T, Phaneuf S. A new approach to flow cytometry-based CD4 T-cell enumeration for absolute and percentage values. 12th World AIDS Conference, Geneva, June 28-July 3. 1998:765. Abstract 41116.

  6. Bergeron M, Phaneuf S, Chabot C, Ding T, Mandy F. Cryopreserved whole blood control preperation for monitoring single platform absolute and ratio count of lymphocyte subsets on clinical flow cytometers. Cytometry 1998;9(Suppl):125.

  7. Bergeron M, Ding T, Shafaie A, Mandy F. Evaluation of the robustness of some commercial stabilized cell preparations under stressful environmental conditions. Can J Infect Dis 1999;10(Suppl B):15B. Abstract A154P.

  8. Bradley J, Mandy F, Fahey J. International program for quality assurance and standardization for immunological measures relevant to HIV/AIDS: QASI program for CD4 proficiency testing. 10th International Congress of Immunology, New Delhi, India, Nov. 1-6, 1998. p. 577.

  9. Chabot C, O'Shaughnessy M, Strathdee S, Nicholson JK, Shenkin M, Mandy F. Evaluation of stressed blood samples with four immunophenotyping gating strategies and 4-colour flow cytometry. Can J Infect Dis 1997;8(Suppl A):73A. Abstract 453.

  10. Chabot C, Minkus T, Bergeron M, Mandy F. 4-color analysis: Comparison between a single laser and a dual laser flow cytometer. Cytometry (Communications in Clinical Cytometry) 1998:34(1):57.

  11. Chabot C, Minkus T, Phaneuf S, Bergeron M, Mandy F. T-cell subsets comparison between two clinical 4-color instruments with automated software. Cytometry (Communications in Clinical Cytometry) 199;38(2):93.

  12. Ding T, Bergeron M, Rud E, Mandy F. Cryopreserved reference material for immunophenotyping T-cell subsets by flow cytometry. XI International Conference on AIDS, Vancouver, July 7-12, 1996. 1996:258. Abstract 4025.

  13. Ding T, Chabot C, Parenteau M, Bergeron M, Mandy F, McCulloch B, Barnabe P. Cryopreserved control material for T-cell subset absolute count enumeration by flow cytometry. Can J Infect Dis 1997;8(Suppl A):72A. Abstract 452.

  14. Ding T, Minkus T, Faucher S, Bergeron M, Mandy F. A rapid and reproducible 4-color protocol for the measurement of Th1 and Th2 type intracellular cytokine expression in whole blood. Can J Infect Dis 1999;10(Suppl B):15B. Abstract A155P.

  15. Fahey JL, Mandy F, Bradley J. International program for quality assurance and standardization for immunological measures relevant to HIV/AIDS: QASI program for CD4 proficiency testing. 12th World AIDS Conference, Geneva, June 28-July 3, 1998:105. Abstract 13101.

  16. Faucher S, Bergeron M, Solajic Z, Mandy F. Inhibition of cytokine secretion by brefeldine A during in vitro lymphocyte activation. Cytometry 1998;(Suppl 9):125.

  17. Faucher S, Fex C, Camilla C, Fert V, Bergeron M, Mandy F, Angel JB. Downregulation of IL-10, TNFa and IFNg secretion by Saquinavir. Can J Infect Dis 1999;10(Suppl B):13B. Abstract A116.

  18. Faucher S, Minkus T, Ding T, Merks H, Camilla C, Fert V, Bergeron M, Mandy F. Secreted and intracellular cytokine levels in whole blood cultures of HIV infected individuals. Cytometry (Communications in Clinical Cytometry) 1999;38(2):93.

  19. Lacroix F, Merks H, Bergeron M, Mandy F, Waxdal M. Evaluation of a commercial kit for flow cytometric assay to measure NK cell activity. Can J Infect Dis 1997;8(Suppl A);73A. Abstract 455.

  20. Lacroix F, Bergeron M, Mandy F. Evaluation of three commercial preserved whole blood controls for flow cytometry. Can J Infect Dis 1997;8(Suppl A):73A. Abstract 454.

  21. Lacroix F, Faucher S, Merks H, Chabot C, Minkus T, Bergeron M, Mandy F. A novel gating approach with ethidium monoazide bromide for intracellular cytokine assay by flow cytometry. Can J Infect Dis 1998;9(Suppl A):56A. Abstract 325P.

  22. Lacroix F, Minkus T, Chabot C, Merks H, Bergeron M, Mandy F. Effects of lysis techniques on absolute cell count using the intracellular measurement of cytokine. Cytometry (Communications in Clinical Cytometry) 1998;34(1):57.

  23. Lapointe N, Merks H, Bergeron M, Roederer M, Mandy F. A 4-color flow cytometric protocol to measure naïve T-cell subsets in frozen samples from HIV infected pregnant women. Can J Infect Dis 1997;8(Suppl A):57A. Abstract 352.

  24. Lenkei R, Gratama JW, Rothe G, D'hautcourt JL, Mandy F, Marti GE. Quantitative flow cytometry 1997. Testing the unified (common) window of analysis (UWA) and reagents, antibodies, and microbeads. Cytometry 1998;(Suppl 9):124.

  25. Mandy F, Bergeron M, Chabot C. A new option for reporting absolute CD4 T-cell count utilizing conventional flow cytometers. Cytometry 1996;(Suppl 8):88.

  26. Mandy F, Bergeron M, Lacroix F, Minkus T. A quantitative flow cytometric method for measuring the kinetics of early CD4 T-cell activation. Cytometry 1996;(Suppl 8):92.

  27. Mandy F, Bergeron M, Chabot C, 42 Participating Laboratories. The Canadian T-cell immunophenotyping quality assurance programme. Cytometry 1996;(Suppl 8):87.

  28. Mandy F, Bradley J, Fahey JL. International programme for quality assurance and standardization for immunological measures relevant to HIV/AIDS: QASI. 10th International Conference on AIDS and STD in Africa. Abidjan, Côte d'Ivoire, 7-11 December, 1997:190. Abstract A648.

  29. Mandy F, Bergeron M, Tsoukas C, Lapointe N, Participating CTN Members, Kidd P, Paxton H, Landay A, Gelman B, Kagan J. Predicting absolute CD4 T-cell numbers from percentage of lymphocytes. Can J Infect Dis 1997;8(Suppl A):74A. Abstract 457.

  30. Mandy F, Bergeron M, Chabot C, Somorjai R, 44 Participating Laboratories Across Canada (CTN). The evolution of a Canadian quality assurance programme for CD4 T-cell enumeration: The impact of frequency of participation. Can J Infect Dis 1997;8(Suppl A):75A. Abstract 463.

  31. Mandy F. Flow cytometry based multiplexed assays of the 21st Century. Cytometry (Communications in Clinical Cytometry) 1999;38(2):87.

  32. Marti GE, Zenger VE, Vogt R, Mandy F, Schwartz A, Lenkei R. Quantitative flow cytometry (QFCM): History,practical theory, consensus and inter-laboratory variation. Cytometry 1998;(Suppl 9):123.

  33. Merks H, Minkus T, Bergeron M, Cameron W, Diaz-Mitoma F, Roederer M, Mandy F. Evaluation of 4-colour combination to measure naïve T-cell subsets using current clinical flow cytometers. Can J Infect Dis 1997;8(Suppl A):73A. Abstract 456.

  34. Merks H, Bergeron M, Mandy F. Comparison of intracellular cytokine measurement in fresh and frozen PBMC's using a flow based assay. Cytometry (Communications in Clinical Cytometry) 1998;34(1):57.

  35. Merzouki A, Estable MC, Acel A, Moniz DE, Montaner JS, Mandy F, Arella M. Sequence variability of HIV-1 ADCC epitopes in vivo V3 loop ADCC epitopes appear to be the major determinants for ADCC activity at different stages of the infection. XI International Conference on AIDS, Vancouver, July 7-12, 1996:275.

  36. Merzouki A, Bouhdoud L, Mandy F, Arella M. Absence of correlation between HIV-1 cellular RNA load and proviral sequence diversity: Cross sectional study. Can J Infect Dis 1998;9(Suppl A):57A. Abstract 331P.

  37. Merzouki A, Bouhdoud L, Mandy F, Arella M. HIV-1 cellular RNA load rise in late stages of HIV disease, but do not correlate with the proviral sequence variability: Cross sectional study representing all stages of infection. 12th World AIDS Conference, June 28-July 3, 1998:14. Abstract 11158.

  38. Minkus T, Lacroix F, Faucher S, Bergeron M, Mandy F. A Canadian inter-laboratory evaluation of a kinetic cytometric method for quantitative measurement of CD69+ T-cell activation. XI International Conference on AIDS, 1996, No. 258.

  39. Minkus T, Chabot C, Bergeron M, Mandy F. Comparison of 3 and 4-colour immunophenotyping data from HIV + and HIV- subjects using a FACSCalibur flow cytometer. Cytometry (Communications in Clinical Cytometry) 1998;34(1):58.

  40. Montpetit M, Gleeson T, Mandy F. Th use of flow cytometry as a rapid multiparametric detection system for molecular biological characterization of HIV. Cytometry (Communications in Clinical Cytometry) 1998;34(1):50.

  41. Montpetit M, Mandy F. An introduction to the merge of molecular biology and flow cytometry. Cytometry (Communications in Clinical Cytometry) 1999;38(2):87.

  42. Nicholson JKA, Bergeron M, Mandy F. An evaluation of three gating strategies on stressed blood samples for immunophenotyping HIV individuals: New options are available with multicolor flow cytometry. XI International Conference on AIDS, Vancouver, July 7-12, 1996:257. Abstract 4021.

  43. O'Gorman MRG, Mandy F, Gelmann.R. Volumetric capillary cytometry (VCC) accuracy and precision of CD4 and CD8 T-cell measurements within and between laboratories. XI International Conference on AIDS, Vancouver, July 7-12, 1996:255. Abstract 3049.

  44. Phaneuf S, Chabot C, Bergeron M, Mandy F. Comparison of T-cell subset determinations using manual and 4-color automated analysis on the EPIC-XL flow cytometer. Cytometry (Communications in Clinical Cytometry) 1999;38(2):94.

  45. Phenix BN, Mandy F, Chambers CB, Angel J, Gallicano K, Hawley-Foss N, Cassol S, Cameron DW, Badley A. Prevention of direct HIV induced T-cell apoptosis by inhibitors of HIV protease. Cytometry (Communications in Clinical Cytometry) 1999;38(2):91.

  46. Read S, Assad S, Merks H, Bergeron M, Mandy F. Monitoring naïve T-cell subsets in HIV infected children using a 4-colour flow cytometer protocol. Can J Infect Dis 1997;8(Suppl A):56A. Abstract 351.

  47. Salzman GC, Mandy FF, Bergeron M. A comparison of two cluster analysis methods on multivariate HIV data. Cytometry (Communications in Clinical Cytometry) 1998;34(1):48.

  48. Shenkin M, Bergeron M, Chabot C, Mandy F. Variation with simultaneous four-color immunophenotyping T-cell subsets with clinical flow cytometers. XI International Conference on AIDS, Vancouver July 7-12, 1996:257. Abstract 4020.

  49. Solajic Z, Faucher S, Bergeron M, Mandy F. A microsphere based flow cytometric assay for cytokine measurement. Can J Infect Dis 1998;9(Suppl A):57A. Abstract 330P.

  50. Solajic Z, Faucher S, Bergeron M, Mandy F. Multiplexed flow cytometry assay for cytokine detection using fluorescent microspheres. Cytometry (Communications in Clinical Cytometry) 1998;34(1):58.

  51. Solajic Z, Mandy F, Bergeron M, Perlmutter N, Shapiro H. Evaluation of six cytometers for low level antibody detection. Cytometry (Communications in Clinical Cytometry) 1999;38(2):94.

  52. Traore Y, Mandy F, Bergeron M, Combe P, Bonard D, Msellati L, Gauthier-Charpentier L, Ouargre A, Diagbouga S, Sanou O, Mandelbrot L, Meda N, Dabis F, Van de Perre. T-cell subset immunophenotyping by flow cytometry: A pilot quality assurance programme (QAP) for West Africa. Can J Infect Dis 1999;10(Suppl B):14B. Abstract A153P.

  53. Van de Perre P, Traore Y, Combe PC, Bonard DB, Mandy FF, Gauthier-Charpentier LG, Diagbouga SD, Meda NH. CD4 T-cell phenotyping by flow cytometry: A quality assurance programme (QAP) for African settings. 12th World AIDS Conference, Geneva, June 28-July 3, 1998:809. Abstract 42182

Date de modification : 2001-06-29

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