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Recent Publications

National Laboratory for HIV Immunology (NLHI)

The National Laboratory for HIV Immunology disseminates information through publication in peer reviewed journals, and through presentations and posters at national and international meetings.

Peer Reviewed Articles:

  1. Bergeron M, Faucher S, Minkus T, Lacroix F, Ding T, Phaneuf S, Somorjai R, Summers R, Mandy F. Impact of unified procedures as implemented in the Canadian Quality Assurance Program for T lymphocyte subset enumeration. Participating Flow Cytometry Laboratories of the Canadian Clinical Trials Network for HIV/AIDS Therapies. Cytometry 1998; 33(2):146-155.

  2. Brando B, Barnett D, Janossy G, Mandy F, Autran B, Rothe G, Scarpati B, D'Avanzo G, D'hautcourt JL, Lenkei R, Schmitz G, Kunkl A, Chianese R, Papa S, Gratama JW. Cytofluorometric methods for assessing absolute numbers of cell subsets in blood. Cytometry 2000; 42(6):327-346.

  3. Gratama JW, D'hautcourt JL, Mandy F, Rothe G, Barnett D, Janossy G, Papa S, Schmitz G, Lenkei R. Flow cytometric quantitation of immunofluorescence intensity: problems and perspectives. European Working Group on Clinical Cell Analysis. Cytometry 1998; 33(2):166-178.

  4. Lenkei R, Gratama JW, Rothe G, Schmitz G, D'hautcourt JL, Arekrans A, Mandy F, Marti G. Performance of calibration standards for antigen quantitation with flow cytometry. Cytometry 1998; 33(2):188-196.

  5. Mandy F, Bergeron M, Izaguirre C. Application Tools for Clinical Flow Cytometry: Gating Parameters for Immunophenotyping. Clin Immunol Newsletter 12[2], 25-32. 1992. Ref Type: Generic

  6. Mandy F, Brando B. Enumeration of absolute cell counts using immunophenotypic techniques. In: Robinson JP, Darzynkiewicz Z, Dean PN, Dressler LG, Rabinovitch PS, Stewart CC, Tanke HJ, Wheeless LL, editors. Current Protocols in Cytometry, Supplement 13 edition. New York: John Wiley & Sons, Inc.; 2000. p 6.8.1-6.8.26.

  7. Mandy FF, Bergeron M, Recktenwald D, Izaguirre CA. A simultaneous three-color T cell subsets analysis with single laser flow cytometers using T cell gating protocol. Comparison with conventional two- color immunophenotyping method. J Immunol Methods 1992; 156(2):151-162.

  8. Mandy FF, Bergeron M, Minkus T. Principles of flow cytometry. Transfus Sci 1995; 16(4):303-314.

  9. Mandy FF, Bergeron M, Minkus T. Evolution of leukocyte immunophenotyping as influenced by the HIV/AIDS pandemic: a short history of the development of gating strategies for CD4+ T-cell enumeration. Cytometry 1997; 30(4):157-165.

  10. Nicholson J, Kidd P, Mandy F, Livnat D, Kagan J. Three-color supplement to the NIAID DAIDS guideline for flow cytometric immunophenotyping. Cytometry 1996; 26(3):227-230.

  11. Nicholson JKA, Mandy FF. Immunophenotyping in HIV infection. In: Stewart C, Nicholson JKA, editors. Immunophenotyping, 1st edition. New York: Wiley-Liss; 2000. p 261-289.

  12. O'Gorman MR, Gelman R. Inter- and intrainstitutional evaluation of automated volumetric capillary cytometry for the quantitation of CD4- and CD8-positive T lymphocytes in the peripheral blood of persons infected with human immunodeficiency virus. Site Investigators and the NIAID New CD4 Technologies Focus Group. Clin Diagn Lab Immunol 1997; 4(2):173-179.

  13. Prud'homme IT, Kim JE, Pilon RG, Minkus T, Hawley-Foss N, Cameron W, Rud EW. Amplicor HIV monitor, NASBA HIV-1 RNA QT and quantiplex HIV RNA version 2.0 viral load assays: a Canadian evaluation. J Clin Virol 1998; 11(3):189-202.

  14. Schnizlein-Bick CT, Spritzler J, Wilkening CL, Nicholson JK, O'Gorman MR. Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults. Site Investigators and The NIAID DAIDS New Technologies Evaluation Group. Clin Diagn Lab Immunol 2000; 7(3):336-343.

  15. Zenger VE, Vogt R, Mandy F, Schwartz A, Marti GE. Quantitative flow cytometry: inter-laboratory variation. Cytometry 1998; 33(2):138-145.

Non-Peer Reviewed Articles in International Journals:

  1. Lenkei R, Marti G, Vogt R, Nicholson J, Mandy F. Meeting Summary: EC, FDA, and Health Canada. Cytometry (Communications in Clinical Cytometry) 33[2], 94. 1998.

  2. Lenkei R, Mandy F, Marti G, Vogt R. Preface. Cytometry (Communications in Clinical Cytometry) 33[2], 93. 1998.

  3. Mandy F. Abstracts from the 8th Canadian Flow Cytometry Consensus Meeting, November 16-18, 1997, Aylmer, Quebec, Canada. Cytometry (Communications in Clinical Flow Cytometry) 34[1], 50. 1998.

  4. Mandy F. Abstracts from the 9th Canadian Flow Cytometry Consensus Meeting, October 25-27,1998, Mont Ste.Anne,Quebec, Canada. Cytometry (Communications in Clinical Cytometry) 38[2], 81-82. 1999.

  5. Mandy F. An Introduction to a Unique Review Article. Cytometry (Communications in Clinical Cytometry) 38[1], 1. 1999.

  6. Mandy F, Bergeron M, Minkus T. Current Activities and Directions on the Use of Flow Cytometry in HIV Disease. Canadian Hematology Society Newletter April. 1998.

  7. Montpetit M, Gleeson T, Mandy F. Flow Molecular Cytobiology: The Next Generation? Clinical Immunology Newsletter 18[11/12]. 1998.

Published Abstracts:

  1. Bagwell CB, Stelzer GT, Purvis N, Schwartz A, Stewart C, Mandy F. Multiple Intensity Method for Compensating Listmode Data. Cytometry 8[Sup], 119. 1996.

  2. Bergeron M, Lacroix F, Minkus T, Chabot C, Phaneuf S, Somorjai R, Mandy F. Comparison of Single Versus Double Platform Absolute Count Determination of T-cell Subsets by Flow Cytometry. XI International Conference on AIDS, 255. 1996.

  3. Bergeron M, Lacroix F, Minkus T, Chabot C, Phaneuf S, Somorjai R, Mandy F. Evaluation of Absolute Count Determinations of CD4 T-cells in HIV Infection Utilizing Various Single and Double Platform Based Cytometric Methods. Cytometry 8[Sup], 77. 1996.

  4. Bergeron M, Phaneuf S, Ding T, Minkus T, Mandy F. Single PLatform Technology: To Evaluate Inter-laboratory Variation. Can J Infect Dis 8[Sup A], 74A. 1997.

  5. Bergeron M, Mandy F, Ding T, Phaneuf S. A New Approach to Flow Cytometry-based CD4 T-cell Enumeration for Absolute and Percentage Values. 12th World AIDS Conference , 765. 1998.

  6. Bergeron M, Phaneuf S, Chabot C, Ding T, Mandy F. Cryopreserved Whole Blood Control Preperation for Monitoring Single Platform Absolute and Ratio Count of Lymphocyte Subsets on Clinical Flow Cytometers. Cytometry 9[sup.], 125. 1998.

  7. Bergeron M, Ding T, Shafaie A, Mandy F. Evaluation of the Robustness of some commercial stabilized cell preparations under stressful environmental conditions. Can J Infect Dis 10 Sup. 1999.

  8. Bradley J, Mandy F, Fahey J. International Program for Quality Assurance and Standardization for Immunological Measures Relevant to HIV/AIDS : QASI Program for CD4 Proficiency Testing. 10th International Congress of Immunology, New Delhi, India, Nov. 1-6, 1998. 1998.

  9. Chabot C, O'Shaughnessy M, Strathdee S, Nicholson JK, Shenkin M, Mandy F. Evaluation of Stressed Blood Samples with Four Immunophenotyping Gating Strategies and 4-Colour Flow Cytometry. Can J Infect Dis 8[Sup A], 73A. 1997.

  10. Chabot C, Minkus T, Bergeron M, Mandy F. 4-Color Analysis: Comparison Between a Single Laser and a Dual Laser Flow Cytometer. Cytometry (Communications in Clinical Cytometry) 34[1], 57. 1998.

  11. Chabot C, Minkus T, Phaneuf S, Bergeron M, Mandy F. T-cell Subsets Comparison Between Two Clinical 4-Color Instruments with Automated Software. Cytometry (Communications in Clinical Cytometry) 38[2], 93. 1999.

  12. Ding T, Bergeron M, Rud E, Mandy F. Cryopreserved Reference Material for Immunophenotyping T-cell Subsets by Flow Cytometry. XI International Conference on AIDS , 258. 1996.

  13. Ding T, Chabot C, Parenteau M, Bergeron M, Mandy F, McCulloch B, Barnabe P. Cryppreserved Control Material for T-cell Subset Absolute Count Enumeration by Flow Cytometry. Can J Infect Dis 8[Sup A], 72A. 1997.

  14. Ding T, Minkus T, Faucher S, Bergeron M, Mandy F. A Rapid and Reproducible 4-Color Protocol for the Measurement of Th1 and Th2 Type Intracellular Cytokine Expression in Whole Blood. Can J Infect Dis 10 Sup. 1999.

  15. Fahey JL, Mandy F, Bradley J. International Program for Quality Assurance and Standardization for Immunological Measures Relevant to HIV/AIDS : QASI Program for CD4 Proficiency Testing. 12th World AIDS Conference , 105. 1998.

  16. Faucher S, Bergeron M, Solajic Z, Mandy F. Inhibition of Cytokine Secretion by Brefeldine A During In Vitro Lymphocyte Activation. Cytometry 9[sup.], 125. 1998.

  17. Faucher S, Fex C, Camilla C, Fert V, Bergeron M, Mandy F, Angel JB. Downregulation of IL-10, TNFa and IFNg secretion by Saquinavir. Can J Infect Dis 10 Sup. 1999.

  18. Faucher S, Minkus T, Ding T, Merks H, Camilla C, Fert V, Bergeron M, Mandy F. Secreted and Intracellular Cytokine Levels in Whole Blood Cultures of HIV Infected Individuals. Cytometry (Communications in Clinical Cytometry) 38[2], 93. 1999.

  19. Lacroix F, Merks H, Bergeron M, Mandy F, Waxdal M. Evaluation of a Commercial Kit for Flow Cytometric Assay to Measure NK Cell Activity. Can J Infect Dis 8[Sup A], 73A. 1997.

  20. Lacroix F, Bergeron M, Mandy F. Evaluation of Three Commercial Preserved Whole Blood Controls for Flow Cytometry. Can J Infect Dis 8[Sup A], 73A. 1997.

  21. Lacroix F, Faucher S, Merks H, Chabot C, Minkus T, Bergeron M, Mandy F. A Novel Gating Approach With Ethidium Monoazide Bromide for Intracellular Cytokine Assay by Flow Cytometry. Can J Infect Dis 9[Suppl A], 56A. 1998.

  22. Lacroix F, Minkus T, Chabot C, Merks H, Bergeron M, Mandy F. Effects of Lysis Techniques on Absolute Cell Count Using the Intracellular Measurement of Cytokine. Cytometry (Communications in Clinical Cytometry) 34[1], 57. 1998.

  23. Lapointe N, Merks H, Bergeron M, Roederer M, Mandy F. A 4-Color Flow Cytometric Protocol to Measure naïve T-cell Subsets in Frozen Samples From HIV Infected Pregnant Women. Can J Infect Dis 8[Sup A], 37A. 1997.

  24. Lenkei R, Gratama JW, Rothe G, D'hautcourt JL, Mandy F, Marti GE. Quantitative Flow Cytometry 1997. Testing the Unified (Common) Window of Analysis (UWA) and Reagents, Antibodies, and Microbeads. Cytometry 9[sup], 124. 1998.

  25. Mandy F, Bergeron M, Chabot C. A New Option for Reporting Absolute CD4 T-cell Count Utilizing Conventional Flow Cytometers. Cytometry 8[Sup], 88. 1996.

  26. Mandy F, Bergeron M, Lacroix F, Minkus T. A Quantitative Flow Cytometric Method for Measuring the Kinetics of Early CD4 T-cell Activation. Cytometry 8[Sup], 92. 1996.

  27. Mandy F, Bergeron M, Chabot C, 42 Participating Laboratories. The Canadian T-cell Immunophenotyping Quality Assurance Programme. Cytometry 8[Sup], 87. 1996.

  28. Mandy F. International Programme for Quality Assurance and Standardization for Immunological Measures Relevant to HIV/AIDS : QASI. 10th International Conference on AIDS and STD in Africa . 1997.

  29. Mandy F, Bergeron M, Tsoukas C, Lapointe N, Participating CTN Members, Kidd P, Paxton H, Landay A, Gelman B, Kagan J. Predicting Absolute CD4 T-cell Numbers From Percentage of Lymphocytes. Can J Infect Dis 8[Sup A], 74A. 1997.

  30. Mandy F. Quality Assurance Issues and Standardization for Immunological Measures Relevant to HIV. 10th International Conference on AIDS and STD in Africa . 1997.

  31. Mandy F, Bergeron M, Chabot C, Somorjai R, 44 Participating Laboratories Across Canada (CTN). The Evolution of a Canadian Quality Assurance Programme for CD4 T-cell Enumeration: the Impact of Frequency of Participation. Can J Infect Dis 8[Sup A], 75A. 1997.

  32. Mandy F. Flow Cytometry Based Multiplexed Assays of the 21st Century. Cytometry (Communications in Clinical Cytometry) 38[2], 87. 1999.

  33. Marti GE, Zenger VE, Vogt R, Mandy F, Schwartz A, Lenkei R. Quantitative Flow Cytometry (QFCM): History,Practical Theory, Consensus and Inter-Laboratory Variation. Cytometry 9[Sup.], 123. 1998.

  34. Merks H, Minkus T, Bergeron M, Cameron W, Diaz-Mitoma F, Roederer M, Mandy F. Evaluation of 4-Colour Combination to Measure Naïve T-cell Subsets Using Current Clinical Flow Cytometers. Can J Infect Dis 8[Sup A], 73A. 1997.

  35. Merks H, Bergeron M, Mandy F. Comparison of Intracellular Cytokine Measurement in Fresh and Frozen PBMC's Using a Flow Based Assay. Cytometry (Communications in Clinical Cytometry) 34[1], 57. 1998.

  36. Merzouki A, Estable MC, Acel A, Moniz DE, Montaner JS, Mandy F, Arella M. Sequence Variability of HIV-1 ADCC Epitopes in vivo V3 Loop ADCC Epitopes Appear to be the Major Determinants for ADCC Activity at Different Stages of the Infection. XI International Conference on AIDS , 275. 1996.

  37. Merzouki A, Bouhdoud L, Mandy F, Arella M. Absence of Correlation Between HIV-1 Cellular RNA Load and Proviral Sequence Diversity: Cross Sectional Study. Can J Infect Dis 9[Suppl A], 57A. 1998.

  38. Merzouki A, Bouhdoud L, Mandy F, Arella M. HIV-1 Cellular RNA Load Rise in Late Stages of HIV Disease, but do not Correlate with the Proviral Sequence Variability :Cross Sectional Study Representing All Stages of Infection. 12th World AIDS Conference, 14. 1998.

  39. Minkus T, Lacroix F, Faucher S, Bergeron M, Mandy F. A Canadian Inter-Laboratory Evaluation of a Kinetic Cytometric Method for Quantitative Measurement of CD69+ T-cell Activation. XI International Conference on AIDS, 258. 1996.

  40. Minkus T, Chabot C, Bergeron M, Mandy F. Comparison of 3 and 4-Colour Immunophenotyping Data From HIV + and HIV- Subjects Using a FACSCalibur Flow Cytometer. Cytometry (Communications in Clinical Cytometry) 34[1], 58. 1998.

  41. Montpetit M, Gleeson T, Mandy F. Th Use of Flow Cytometry as a Rapid Multiparametric Detection System for Molecular Biological Characterization of HIV. Cytometry (Communications in Clinical Cytometry) 34[1], 50. 1998.

  42. Montpetit M, Mandy F. An Introduction to the Merge of Molecular Biology and Flow Cytometry. Cytometry (Communications in Clinical Cytometry) 38[2], 87. 1999.

  43. Nicholson JKA, Bergeron M, Mandy F. An Evaluation of Three Gating Strategies on Stressed Blood Samples for Immunophenotyping HIV Individuals : New Options are Available with Multicolor Flow Cytometry. XI International Conference on AIDS , 257. 1996.

  44. O'Gorman MRG, Mandy F, Gelmann.R. Volumetric Capillary Cytometry (VCC) Accuracy and Precision of CD4 and Cd8 T-cell Measurements Within and Between Laboratories. XI International Conference on AIDS , 255. 1996.

  45. Phaneuf S, Chabot C, Bergeron M, Mandy F. Comparison of T-cell Subset Determinations Using Manual and 4-color Automated Analysis on the EPIC-XL Flow Cytometer. Cytometry (Communications in Clinical Cytometry) 38[2], 94. 1999.

  46. Phenix BN, Mandy F, Chambers CB, Angel J, Gallicano K, Hawley-Foss N, Cassol S, Cameron DW, Badley A. Prevention of Direct HIV Induced T-cell Apoptosis by Inhibitors of HIV Protease. Cytometry (Communications in Clinical Cytometry) 38[2], 91. 1999.

  47. Read S, Assad S, Merks H, Bergeron M, Mandy F. Monitoring Naïve T-cell Subsets in HIV Infected Children Using a 4-Colour Flow Cytometer Protocol. Can J Infect Dis 8[Sup.A], 36A. 1997.

  48. Salzman GC, Mandy FF, Bergeron M. A Comparison of Two Cluster Analysis Methods on Multivariate HIV Data. Cytometry (Communications in Clinical Cytometry) 34[1], 48. 1998.

  49. Shenkin M, Bergeron M, Chabot C, Mandy F. Variation with Simultaneous Four-Color Immunophenotyping T-cell Subsets with Clinical Flow Cytometers. XI International Conference on AIDS , 257. 1996.

  50. Solajic Z, Faucher S, Bergeron M, Mandy F. A Microsphere Based Flow Cytometric Assay for Cytokine Measurement. Can J Infect Dis 9[(Suppl A)], 57A. 1998.

  51. Solajic Z, Faucher S, Bergeron M, Mandy F. Multiplexed Flow Cytometry Assay for Cytokine Detection Using Fluorescent Microspheres. Cytometry (Communications in Clinical Cytometry) 34[1], 58. 1998.

  52. Solajic Z, Mandy F, Bergeron M, Perlmutter N, Shapiro H. Evaluation of Six Cytometers for Low Level Antibody Detection. Cytometry (Communications in Clinical Cytometry) 38[2], 94. 1999.

  53. Traore Y, Mandy F, Bergeron M, Combe P, Bonard D, Msellati L, Gauthier-Charpentier L, Ouargre A, Diagbouga S, Sanou O, Mandelbrot L, Meda N, Dabis F, Van de Perre. T-cell Subset Immunophenotyping by Flow Cytometry: A Pilot Quality Assurance Programme (QAP) for West Africa. Can J Infect Dis 10 Sup. 1999.

  54. Van de Perre P, Traore Y, Combe PC, Bonard DB, Mandy FF, Gauthier-Charpentier LG, Diagbouga SD, Meda NH. CD4 T-cell Phenotyping by Flow Cytometry: A Quality Assurance Programme (QAP) for African Settings. 12th World AIDS Conference , 809. 1998.

Date Modified: 2001-06-29

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